Web10 mobility of the tethered probes relative to the DNA, the FAM/TAMRA dyes were positioned in their mean positions relative to the B-DNA helix as determined for the structurally similar Alexa 488 fluorophore by Sindbert et al. using MD simulations.[13] In this respect, the mean position is not necessarily the most probable WebDNA-2A Target 3’-AAT GTA TCC AT-5’-TAMRA Table S1: Sequences of nucleic acids used in this study. S3 Single Molecule Imaging Data Analysis Images were analyzed using a super-resolution imaging scheme previously described.26 First, we located all fluorescent spots in each image, and fit a 2D Gaussian to each spot to locate
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